|
KMID : 0894520030070010009
|
|
Development & Reproduction
2003 Volume.7 No. 1 p.9 ~ p.13
|
|
|
Effect of Diluents on the Cold Storage of Sperm in Sea Urchin, Hemicentrotus pulcherrimus
|
|
고강희/Kho KH
강경호/ 김재민/Kang KH/Kim JM
|
|
|
Abstract
|
|
|
|
A series of experiments were conducted to compare the effects of various diluents in cold storage on the sea urchin, Hemicentrotus pulcherimus sperm. Various diluents of glucose solutions, artificial sea water(ASW) and 50% ASW were used to store the sperm at 4?C. The storage effect was evaluated using sperm activity index(SAI), survival rate of sperm and fertilization rate to egg. ASW and 1.2 M glucose were found to be better diluents which maintained high motility and survival rate of sperm f3r a storage period of 30 days. Optimal pH of diluent to store the sperm at 4?C is 7.0∼8.0. In order to keep high SAI and survival rate of sperm, addition of 400 ppm neomycin into the diluent revealed the best storage results.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|